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small molecule wnt activation (Funakoshi ltd)

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Funakoshi ltd small molecule wnt activation
Small Molecule Wnt Activation, supplied by Funakoshi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/small+molecule+wnt+activation/small+molecule+wnt+activation/pm40614731-344-1-22
Average 90 stars, based on 1 article reviews

small molecule wnt activation - by Bioz Stars, 2026-11

90/100 stars

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Activation Assay:

Article Title: Developmental cues from epicardial cells simultaneously promote cardiomyocyte proliferation and electrochemical maturation.
Article Snippet: .. Interestingly, small molecule WNT activation has also been used to induce expression of the compact myocardium markers HEY2 and FZD2 in hiPSC-CM (Funakoshi et al., 2021). .. These markers, along with increased CM proliferation, were both significantly increased here in the CM co-cultured with EPCs (Funakoshi et al., 2021).

Expressing:

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The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, IWP2 (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. ). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.

Journal: Cell Death Discovery

Article Title: Transcriptome-based prediction of drugs, inhibiting cardiomyogenesis in human induced pluripotent stem cells

doi: 10.1038/s41420-023-01616-6

Figure Lengend Snippet: The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, IWP2 (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. ). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.

Article Snippet: On day 0, the differentiation was induced by adding 10 µM Wnt activator small molecule CHIR (R&D Systems, Minneapolis, USA) in RPMI 1640 GlutaMAX™ medium (Thermo Fisher Scientific, Germany) plus B-27™ Supplement, minus insulin (Thermo Fisher Scientific, Germany).

Techniques: Cell Culture